JEV entry depends on clathrin. PK15 cells were transfected with siRNA targeting clathrin heavy-chain (siCHC) or non-targeting siRNA (siCtrl) and infected with JEV at 48 h post-transfection. 36 hpi. (A) The percentage of internalized viruses of transfected cells was determined by flow cytometry and normalized to the value for the siCtrl. The results are presented as the mean ± SD. (B) JEV E protein was analyzed by western blot analysis. β-actin was used as an internal loading control. -, control without JEV infection. The mean densities of the protein bands are shown as a bar graph under the immunoblot. (C) Clathrin knockdown cells were analyzed at 48 h post-transfection by the immunoblotting for clathrin. The mean densities of the protein bands are shown as a bar graph under the immunoblot. **, p<0.01.