Virology Journal

Table 2 Unique amino acid changes in American genotype DENV2 (parental strains QR94 and PR159, PR159-derived midgut escape isolate EM41 and QR94-derived midgut escape isolate EM33) based on BLAST searches in NCBI GenBank

From: Isolation of midgut escape mutants of two American genotype dengue 2 viruses from Aedes aegypti

Virus	C	prM	E	NS1	NS2a	NS2b	NS3	NS4a	NS4b	NS5
QR94		E89G	T55S	I247Y	T25S		A373T			N174D
				L247Y						K469R
				F247Y						K469R
EM33		E89G	T55S	I247Y	T25S		A373T			N174D
			E202K	L247Y						K469R
			E202K	F247Y						K469R
PR159			E202K	M123T	A88T					S631N
EM41			Q77E	M123T	A88T		E93D			S631N
EM41			E202K	M123T	A88T		E93D			S631N

In bold: additional amino acid substitutions identified in the midgut escape isolates EM33 and EM41, which were absent in the parental viruses.

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ISSN: 1743-422X

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