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Table 2 ACLSV and CGRMV genomic RNA copy numbers in three peach tissues

From: A duplex, SYBR Green I-based RT-qPCR assay for the simultaneous detection of Apple chlorotic leaf spot virus and Cherry green ring mottle virus in peach

 

Ct value (X ± S.D.)a [Number of copies (X ± S.E.)]b

 

ACLSV

CGRMV

RPII

UBQ10

Leaf

23.32 ± 0.24c [1.12 × 106 ± 1.82 × 105]

18.66 ± 0.23f [4.07 × 108 ± 4.17 × 107]

17.16 ± 0.23i [2.45 × 109 ± 2.64 × 108]

18.67 ± 0.18l [3.39 × 109 ± 3.02 × 108]

Bark

22.38 ± 0.36d [2.14 × 106 ± 5.13 × 105]

18.18 ± 0.30g [5.62 × 108 ± 6.92 × 107]

17.46 ± 0.44j [2.04 × 109 ± 4.16 × 108]

19.02 ± 0.30m [2.68 × 109 ± 3.89 × 108]

Flower

19.61 ± 0.35e [1.38 × 107 ± 3.31 × 106]

19.37 ± 0.40h [2.51 × 108 ± 6.92 × 107]

17.76 ± 0.49k [1.68 × 109 ± 3.78 × 108]

19.90 ± 0.41n [1.47 × 109 ± 2.91 × 108]

  1. aAverage threshold cycle (Ct) and standard deviation (S.D.) obtained from 33 different samples.
  2. bAverage number of RNA copies (X) per sample and standard error (S.E.).
  3. c-nCoefficient of variation (CV%) between assays: c = 1.02, d = 1.59, e = 1.76, f = 0.80, g = 1.59, h = 1.76, i = 0.70, j = 1.35, k = 1.51, l = 0.57, m = 0.95, n = 1.33.