Figure 2From: A duplex, SYBR Green I-based RT-qPCR assay for the simultaneous detection of Apple chlorotic leaf spot virus and Cherry green ring mottle virus in peachMelting curve analysis for duplex RT-qPCR (A), single RT-qPCR (B and D), and no template RT-qPCR (C) as the negative control tested for ACLSCV and/or CGRMV. Figure 2C shows the melting curve assay for primer pairs AC62F/AC62R and CG732F/CG732R; RNA from healthy peach tissue was used as a template.Back to article page