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Figure 1 | Virology Journal

Figure 1

From: A duplex, SYBR Green I-based RT-qPCR assay for the simultaneous detection of Apple chlorotic leaf spot virus and Cherry green ring mottle virus in peach

Figure 1

Standard curves for SYBR Green I-based real-time RT-PCR amplification of standard ACLSV, CGRMV, RPII and UBQ10 RNA with specific primer pairs (see Table1). Amplification plots showing the testing in duplicate of a 10-fold dilution series containing (A) standard ACLSV RNA from 1.08 × 109 to 1.08 × 102 template copies/reaction, (B) standard CGRMV RNA from 1.13 × 1010 to 1.13 × 104 template copies/reaction, (C) standard RPII RNA from 2.09 × 1013 to 2.09 × 108 template copies/reaction, and (D) standard UBQ10 RNA from 1.90 × 1012 to 1.90 × 106 template copies/reaction.

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