Detection of SCARB2 expression in SCARB2-overexpressing and parental cells. (a) Relative SCARB2 mRNA level was detected by real-time RT-PCR with β-actin as the internal control. (b) SCARB2 protein of six cells was detected by Western blot using an anti-SCARB2 antibody. (c) Cell surface expression of SCARB2 in six cells. Three parental cells were stained with an anti-SCARB2 antibody (solid lines), SCARB2 cells were stained with an anti-SCARB2 antibody (grey region) or a secondary antibody alone (dotted lines) and analyzed by flow cytometry.