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Figure 5 | Virology Journal

Figure 5

From: Actin filaments disruption and stabilization affect measles virus maturation by different mechanisms

Figure 5

Influence of cytochalasin D treatment on MV glycoprotein-mediated cell-to-cell fusion. ( A ) MDCK cells were infected with MV at an MOI of 10 in the presence of a fusion-inhibitory peptide (FIP). CD was added at 12 h p.i., and cells were washed, detached and mixed with non-infected and non-inhibitor-treated Vero cells at 48 h p.i.. 5 h later, syncytium formation was visualized by Giemsa staining. ( B ) Cells were cotransfected in the presence of FIP with plasmids encoding the MV glycoproteins H and F. CD was added at 6 h post transfection, and cells were washed and detached at 24 h post transfection. 5 h after mixing with non-treated Vero cells, syncytia formation was visualized by Giemsa staining. In the upper panels, a syncytium is exemplarily shown. Fusion was quantified as described in the methods and is shown in the lower panels. Error bars indicate standard error of the mean (SEM). ** P < 0.01.

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