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Figure 1 | Virology Journal

Figure 1

From: Actin filaments disruption and stabilization affect measles virus maturation by different mechanisms

Figure 1

Influence of F-actin disruption and stabilization on the actin cytoskeleton and microtubules. MDCK cells were incubated with the inhibitors (+CD, +Jaspla) which were added at 12 h after seeding. ( A ) For actin staining at 48h p.s., cells were fixed with PFA and permeabilized with Triton X-100. F-actin was detected by phalloidin-FITC. Images were obtained using a confocal laser scanning microscope (Zeiss LSM510). Lower panels show higher magnification of the boxed areas in upper panels. ( B ) For microtubule staining at 48 h p.s., cells were fixed with methanol/acetone and incubated with a monoclonal anti-α-tubulin antibody and fluorophore-conjugated secondary antibodies. Images were obtained using a fluoresence microscope (Zeiss Axiovert 200 M). Bars 10 μm.

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