Table 2 Primers used for RT-PCR and sequencing of Sing-M105-02 and Sing-M100-02 virus isolates
From: A highly divergent Encephalomyocarditis virus isolated from nonhuman primates in Singapore
Primer designation | Sequence (5′ to 3′) | Nucleotide position | Forward/Reverse | Primer reference |
|---|---|---|---|---|
| Â | Â | (estimated) | Â | Â |
EMCVF445 | TTTGCAGGCAGCGGAATC | 490 | Forward | This study |
M105R524 | CGTGCCGCCTTTGCAGGTGTCTG | 524 | Reverse | This study |
M105R870 | CCATTTCTGTATTGCAGGGCAGAGC | 870 | Reverse | This study |
EMCVF1070 | TAATGCAGCCGGGTCTGACC | 1070 | Forward | This study |
M105R2369 | CAAAATAACTGAGTTTGGAC | 2369 | Reverse | This study |
EMCV2737R | GCTACAAAATCTGGAGTAGCA | 2689 | Reverse | This study |
EMCV-P10 | TGCCTATCTCACCTGCCCCCTGGAG | 2674 | Forward | [26] |
M105R2843 | ACTGTGGTCCTGGTGTCA | 2800 | Reverse | This study |
M105VP1F419 | AATCATGGCTTAGCTG | 3000 | Forward | This study |
M105VP1F540 | CCGGAACCTCAAACCA | 3200 | Forward | This study |
M105VIPR768 | ACAGTAGGTCTCGGACA | 3354 | Reverse | This study |
EMCV-P9 | ATCAAGACTCCAGCTCTCGGGGTCA | 3529 | Reverse | [26] |
M105Middle-F505 | AACCCGTGGAAGAGAACCT | 3710 | Forward | This study |
EMC-2B65R | TCGGCAGTAGGGTTTGAG | 3975 | Reverse | [26] |
M105F4204 | ATTGCAGGAATGACAATT | 4200 | Forward | This study |
M105Contiq3-R335 | AGTTGCAGGGTTTTTGGTG | 5500 | Reverse | This study |
M105Contiq3-F327 | CCTGCAACTGCTGGATGT | 5839 | Forward | This study |
M105F5906 | ACAGGAAAAGATACCGATGT | 5980 | Forward | This study |
M105R6000 | TAATGGTCCTGAATTAAG | 6000 | Reverse | This study |
M105R6500 | ATCGAATTTAGACAACACTGC | 6500 | Reverse | This study |
M105POLF16 | ACACTAGATGATGTAGTTT | 7000 | Forward | This study |
M105POLR158 | GTCACTGAGGTGAGTT | 7460 | Reverse | This study |
EMCV-P1 | CCCTACCTCACGGAATGGGGCAAAG | 7631 | Reverse | [13] |
EMCV-P2 | GGTGAGAGCAAGCCTCGCAAAGACAG | 7370 | Forward | [13] |