A highly divergent Encephalomyocarditis virus isolated from nonhuman primates in Singapore

Table 1 Sequences and references for primers used for RT and PCR reactions

Virus designation	Primer names and sequences (5′ to 3′)	Gene target	Amplicon size	References
EMCV	P1 = CCC TAC CTC ACG GAA TGG GGC AAA G	3D polymerase gene	286 bp	[13]
EMCV	P2 = GGT GAG AGC AAG CCT CGC AAA GAC AG	3D polymerase gene	286 bp	[13]
EMCV	P9 = ATC AAG ACT CCA GCT CTC GGG GTC A	VP3/VP1 capsid gene	855 bp	[26]
EMCV	P10 = TGC CTA TCT CAC CTG CCC CCT GGA G	VP3/VP1 capsid gene	855 bp	[26]
Enterovirus	RotbartFor = CCT CCG GCC CCT GAA TGC GGC TAAT	5′ non-coding region	153 bp	[25]
Enterovirus	RotbartRev = ACC GAC GAA TAC CAC TGT TA	5′ non-coding region	153 bp	[25]
Enterovirus	EV2 = TCC GGC CCC TGA ATG CGG CTA ATC C	5′ non-coding region	113 bp	[24]
Enterovirus	EV1 = ACA CGG ACA CCC AAA GTA GTC GGT CC	5′ non-coding region	113 bp	[24]

ISSN: 1743-422X