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Table 3 Determination of cut-offs of indirect ELISAs based on recombinant NP and HA1 proteins

From: Distinction of subtype-specific antibodies against European porcine influenza viruses by indirect ELISA based on recombinant hemagglutinin protein fragment-1

Serum samples Values Recombinant antigens
   NP H1N1av H1N2 N1N1pdm H1pdmN2 H3N2
Negative sera Mean 0,11 0,03 0,06 0,03 0,04 0,14
N = 75 SD 0,08 0,03 0,10 0,08 0,07 0,14
  X + 2SD 0,35* 0,08 0,25 0,19 0,18 0,41
Positive standard Mean 0,42 094 0,61 0,92 0,54 0,58
  SD 0,02 0,01 0,01 0,01 0,02 0,02
Negative standard Mean 0,12 0,06 0,15 0,18 0,16 0,15
  SD 0,02 0,01 0,00 0,01 0,03 0,01
  1. Viruses used for production of recombinant proteins:
  2. NP, H1N1av – A/swine/Germany/R1738/2010.
  3. H1N2 – A/swine/Germany/R1207/2010.
  4. H1N1pdm – A/Germany/R26/2011.
  5. H1pdmN2 – A/swine/Germany/R2035/2011.
  6. H3N2 – A/swine/Germany/R96/2011.
  7. * - For NP a cut-off of 3 SD was used.