Virology Journal

Table 3 Determination of cut-offs of indirect ELISAs based on recombinant NP and HA1 proteins

From: Distinction of subtype-specific antibodies against European porcine influenza viruses by indirect ELISA based on recombinant hemagglutinin protein fragment-1

Serum samples	Values	Recombinant antigens
		NP	H1N1av	H1N2	N1N1pdm	H1pdmN2	H3N2
Negative sera	Mean	0,11	0,03	0,06	0,03	0,04	0,14
N = 75	SD	0,08	0,03	0,10	0,08	0,07	0,14
	X + 2SD	0,35*	0,08	0,25	0,19	0,18	0,41
Positive standard	Mean	0,42	094	0,61	0,92	0,54	0,58
	SD	0,02	0,01	0,01	0,01	0,02	0,02
Negative standard	Mean	0,12	0,06	0,15	0,18	0,16	0,15
	SD	0,02	0,01	0,00	0,01	0,03	0,01

Viruses used for production of recombinant proteins:
NP, H1N1av – A/swine/Germany/R1738/2010.
H1N2 – A/swine/Germany/R1207/2010.
H1N1pdm – A/Germany/R26/2011.
H1pdmN2 – A/swine/Germany/R2035/2011.
H3N2 – A/swine/Germany/R96/2011.
* - For NP a cut-off of 3 SD was used.

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ISSN: 1743-422X

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