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Table 2 HI titres of porcine and ferret post infection sera used in Western blotting and indirect ELISA (homologous serum-antigen pairs depicted in bold)

From: Distinction of subtype-specific antibodies against European porcine influenza viruses by indirect ELISA based on recombinant hemagglutinin protein fragment-1

Serum raised against

Subtype

Host

Hemagglutination inhibiting titre

   

H1N1ava

H1N2b

H1N1pdmc

H1pdmN2d

H3N2e

A/swine/Belzig/2/2001

H1N1av

Swine (OM8)

4*

1

2

1

1

A/swine/Germany/ R681/2011

H1N1av

Ferret (EL1)

6

1

6

2

2

A/swine/Bakum/1832/ 2000

H1N2

Swine (OM1)

1

5

1

1

1

A/swine/Bayern/74/ 2009

H1N1pdm

Swine (OM46)f

4

1

6

5

1

A/swine/Germany/ R2035/2011

H1pdmN2

Swine (OM15/4)

1

3

5

6

1

A/swine/Belzig/54/ 2001

H3N2

Swine (R4)

1

1

1

1

6

A/swine/Germany/ R655/2012

H3N2

Ferret (EL1)

1

1

2

2

9

Unknown, field serum

Pan

swine

6

6

5

5

6

Unknown, field serum

None

swine

1

1

1

1

1

  1. Viruses that had been used for production of recombinant HA1 proteins were also used in HI assays:
  2. a – A/swine/Germany/R1738/2010.
  3. b – A/swine/Germany/R1207/2010.
  4. c – A/Germany/R26/2011.
  5. d – A/swine/Germany/R2035/2011.
  6. e – A/swine/Germany/R96/2011.
  7. *– Log2 titre (starting dilution 1:10).