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Table 2 HI titres of porcine and ferret post infection sera used in Western blotting and indirect ELISA (homologous serum-antigen pairs depicted in bold)

From: Distinction of subtype-specific antibodies against European porcine influenza viruses by indirect ELISA based on recombinant hemagglutinin protein fragment-1

Serum raised against Subtype Host Hemagglutination inhibiting titre
    H1N1ava H1N2b H1N1pdmc H1pdmN2d H3N2e
A/swine/Belzig/2/2001 H1N1av Swine (OM8) 4* 1 2 1 1
A/swine/Germany/ R681/2011 H1N1av Ferret (EL1) 6 1 6 2 2
A/swine/Bakum/1832/ 2000 H1N2 Swine (OM1) 1 5 1 1 1
A/swine/Bayern/74/ 2009 H1N1pdm Swine (OM46)f 4 1 6 5 1
A/swine/Germany/ R2035/2011 H1pdmN2 Swine (OM15/4) 1 3 5 6 1
A/swine/Belzig/54/ 2001 H3N2 Swine (R4) 1 1 1 1 6
A/swine/Germany/ R655/2012 H3N2 Ferret (EL1) 1 1 2 2 9
Unknown, field serum Pan swine 6 6 5 5 6
Unknown, field serum None swine 1 1 1 1 1
  1. Viruses that had been used for production of recombinant HA1 proteins were also used in HI assays:
  2. a – A/swine/Germany/R1738/2010.
  3. b – A/swine/Germany/R1207/2010.
  4. c – A/Germany/R26/2011.
  5. d – A/swine/Germany/R2035/2011.
  6. e – A/swine/Germany/R96/2011.
  7. * Log2 titre (starting dilution 1:10).