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Figure 1 | Virology Journal

Figure 1

From: Distinction of subtype-specific antibodies against European porcine influenza viruses by indirect ELISA based on recombinant hemagglutinin protein fragment-1

Figure 1

Detection of biotinylated recombinant HA1 hemagglutinin and nucleocapsid proteins of European porcine influenza viruses. An amount of approximately 20 μg of recombinant protein was loaded onto each lane and subsequently probed by an anti-biotin monoclonal antibody (A), H1N1pdm (Bavaria/74) specific porcine serum OM46 (B) H1N1av (R681/11) specific ferret serum EL1 (C), H1N2 (Bakum/1800)specific porcine serum OM1 (D) or H3N2 (R655/12) specific ferret serum EL2 (E) as described in materials and methods. Recombinant antigens in lanes: 1 – R26/11 H1N1pdm, 2 – R2035/11 H1pdmN2, 3 – R1207/10 H1N2, 4 – R1738/11 H1N1av, 5 – R1931/11 H1N2, 6 – R76/11 H3N2, 7 – R96/11 H3N2, 8 - nucleocapsid protein of A/swine/Germany/R96/2011 (H3N2), N - Bacterial lysate of Rosettagami cells carrying the BirA plasmid and an empty pET19b vector. The approximate molecular weight of recombinant HA1 (38 kD) and NP (56 kD) is indicated.

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