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Figure 4 | Virology Journal

Figure 4

From: West Nile virus methyltransferase domain interacts with protein kinase G

Figure 4

WNV NS5, but not tick-borne LGTV NS5, interacts with PKGIβ. HEK293T cells were transfected with DNA plasmids encoding WNV NS5 or LGTV NS5 and PKGIβ-Flag. Total protein from the lysate was subjected to immunoprecipitation using α-Flag. Total cell lysates or immunoprecipitated proteins were subjected to Western blot analysis using α-WNV MTase (for the detection of WNV NS5 (left panel)) and α-HA (for the detection of LGTV NS5 (right panel)), α-Flag (for the detection of PKG), and α-actin to show equal protein loading for the lysate.

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