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Figure 3 | Virology Journal

Figure 3

From: West Nile virus methyltransferase domain interacts with protein kinase G

Figure 3

PKG expression is associated with an enhancement in viral production. (a) Expression of PKG in stably transfected BHK-21 cells containing the empty vector (BHK + EV, labeled as EV) or vector expressing PKG (BHK + PKG, labeled as PKG). Cell lysates were subjected to Western blot analysis using α-Flag and α-actin. Western blots show the expression of PKG in BHK + PKG, but not BHK + EV, stable cell lines. (b) The relative level of PKG activity in each BHK stable cell line. PKG activity was assayed in each stably transfected cell lysate by measuring the phosphorylation of a canonical PKG peptide substrate. (c) Effect of PKG on WNV production. The stably transfected BHK cell lines were infected with WNV. Viral production in PFU/ml was measured in quadruplicate by plaque-forming assay at various time-points pi. The figure is a representative experiment of one of three independent experiments. Significant P-values between BHK + EV and BHK + PKG are indicated as follows: *, P< 0.05.

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