Table 1 Primers for PCR amplification of survival gene cDNAs
From: Engineering lentiviral vectors for modulation of dendritic cell apoptotic pathways
ÉcDNA | Primer direction | Primer sequence |
|---|---|---|
c-FLIPS | Forward | 5’- CGCGATATCGCCACCATGGCCCAGAGCCCTGTGTCTG - 3’ |
Reverse | 5’- ATAAGAATGCGGCCGCTCATGCTGGTACTCCATACACTGGCTC - 3’ | |
c-FLIPL | Forward | 5’- GAAGGCCTGCCACCATGGCCCAGAGCCCTGTGTCT - 3’ |
Reverse | 5’- ATAAGAATGCGGCCGCTCACGTAGGAGCCAGGATGAGTTTC - 3’ | |
Bcl-XL | Forward | 5’- CGCGATATCGCCACCATGTCTCAGAGCAACCGGGAGC - 3’ |
Reverse | 5’- ATAAGAATGCGGCCGCTCACTTCCGACTGAAGAGTGAGCC - 3’ | |
M11L | Forward | 5’- AGGCCTGCCACCATGATGTCTCGTTTAAAGACGGCCG - 3’ |
Reverse | 5’- GCGGCCGCCTAGGTCCCTCGGTACCATTTTAGTATTCC - 3’ | |
AKT-1 | Forward | 5’- GATATCGCCACCATGAACGACGTAGCCATTGTGAAGG - 3’ |
Reverse | 5’- GCGGCCGCTCAGGCTGTGCCACTGGCTG - 3’ |