Figure 4

Enforced expression of survival factors protect DC2.4 cells from targeted apoptosis. A) DC2.4 cell lines were cultured in either 2% FCS or 10% FCS-containing media to assess the protective effect of survival factors. B) DC2.4 cell lines were treated with either anti-CD95 or isotype control antibodies to assess the protective effect of survival factor expression on activation of the extrinsic cycle of apoptosis. C) DC2.4 cell lines were treated with or without STS to assess the protective effect of survival factors from the activation of the intrinsic cycle of apoptosis. Cell death in all experiments was analyzed by Annexin V/PI flow cytometry assay and values were normalized to apoptotic cell death (Annexin V+/PI+ double-positive populations) observed in 10% FCS-containing media. Each treatment group was performed in triplicate. Each experiment was performed at least twice independently and representative data are shown with error bars representing standard deviations. *p values <0.05 were designated as significant (*).