Figure 3
From: Engineering lentiviral vectors for modulation of dendritic cell apoptotic pathways
Stable genetic modification of the DC2.4 cell line. A) Flow cytometry plots illustrating expression of rHER-2 in LV-transduced DC2.4 cells. B) Enforced expression of survival genes is shown by immuno-blotting of protein extracts from transduced DC2.4 cells. C) Transduced DC2.4 cells were analyzed for WPRE copy numbers per ng of genomic DNA to assess proviral integration. Each DNA sample was tested in triplicate and mean values are shown. Experiment was performed once with error bars representing standard deviations.