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Figure 2 | Virology Journal

Figure 2

From: Simultaneous detection and differentiation of Rice black streaked dwarf virus (RBSDV) and Southern rice black streaked dwarf virus (SRBSDV) by duplex real time RT-PCR

Figure 2

Optimization of duplex RT-qPCR The concentration of template of RBSDV or SRBSDV were 105 copies. The arrow indicated positive signal (P), otherwise, negative single(N). A: Specific for RBSDV-P probe. P:Standard sample of RBSDV as template; RBSDV-F/R/P as primer and probe. N: Standard sample of RBSDV or H2O as template; RBSDV-F/R as primer; SRBSDV-P as probe. B: Specific for SRBSDV-P probe. P:Standard sample of SRBSDV as template; SRBSDV-F/R/P as primer and probe. N: Standard sample of SRBSDV or H2O as template; SRBSDV-F/R as primer; RBSDV-P as probe. C: Specific for RBSDV-F/R primer. P:Standard sample of RBSDV as template; RBSDV-F/R as primer and RBSDV-P as probe. N: Standard sample of RBSDV or H2O as template; SRBSDV-F/R as primer; RBSDV-P as probe. D: Specific for SRBSDV-F/R primer. P:Standard sample of SRBSDV as template; SRBSDV-F/R as primer and SRBSDV-P as probe. N: Standard sample of SRBSDV or H2O as template; RBSDV-F/R as primer; SRBSDV-P as probe. E, F: Optimization concentration of primer and probe (E) P: standard sample of RBSDV as template. concentration of upstream, downstream primer and probe at a)200 nM 200 nM 400 nM; b) 200 nM 200 nM 200 nM; c) 100 nM 100 nM 200 nM. No positive signal (d): H2O as template. (F) positive: standard sample of SRBSDV as template. concentration of upstream, downstream primer and probe at a) 200 nM 200 nM 400 nM; b) 100 nM 100 nM 200 nM; c) 200 nM 200 nM 200 nM. No positive signal (d): H2O as template.

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Virology Journal

ISSN: 1743-422X