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Table 2 Primer/probe sequences used in gene expression assays

From: Aberrant placental immune parameters in the feline immunodeficiency virus (FIV)-infected cat suggest virus-induced changes in T cell function

Gene

Primer/Probe

Sequence (5′-3′)

β-actin

Probe

/56-FAM/ATG GAG AAG /ZEN/ATCTGGCACCACACCTT/31ABkFQ/

Forward

ATCGAACACGGC ATT GTCACCAAC

Reverse

AGT CAT CTT CTC ACGGTTGGCCTT

IL-1β

Probe

/56′FAM/AAA GAG CCT /ZEN/GGTGCTGTCTGG CTC AGA/31ABkFQ/

Forward

TGATGCAGC CAT ACAGTCACAGGA

Reverse

CAAAGCTCATGCGGAACACCACTT

IL-2

Probe

/56-FAM/ACT CGTCAC /ZEN/AAA CAG TGCACCTGCTT/31ABkFQ/

Forward

TAACCTCAA CTC CTGCCACCACAA

Reverse

TTG CTC CAG CTGTTGCTGTGTTTC

IL-6

Probe

/56-FAM/AACCGT AGA /ZEN/AGTTGGCCTGCAGCT AA/31ABkFQ/

Forward

TCC AGA TGCTGAAGCGTAAGGGAA

Reverse

AAGGGT GAG GTG ATT GTTGTGTGC

IL-10

Probe

/56-FAM/ACCAGGTCC /ZEN/TTGCTG GAG GACTTT AA/31ABkFQ/

Forward

TTTCAAACCAAGGAC GAG CTGCAC

Reverse

AACTGGATCATCTCGGACAAGGCT

IL-17a

Probe

/56-FAM/GTGAGGACA /ZEN/GGA ATA GCATTCCCA CA/31ABkFQ/

Forward

TCC CAT TCAGTT CCC ATC ACT GCT

Reverse

TCGGTAGTTGGG CAT CCTGGATTT

TGF-β

Probe

/56-FAM/AGCAATAAT /ZEN/TCCTGGCGCTAC CTC AGC A/31ABkFQ/

Forward

AGCACGTGGAGCTGTACC AGA AAT

Reverse

TCCAGTGACATC AAA GGA CAG CCA

Gag

Probe

/56-FAM/AAGAACAAC /ZEN/AAG CAG AAGCAA GAT TTGCAC/31ABkFQ/

Forward

CAG GGTGCGCTG CAG ATA AAG AAA

Reverse

ATG CTC TAC ACT GCATCC TAG CTG

FoxP3

Probe

/56-FAM/AGC CTA CAC /ZEN/AAA TGCTTTGTGCGG GT/31ABkFQ/

Forward

GGTTCACACGCATGTTTGCCTTCT

Reverse

ACT CGA ATT CAT CCACGGTCCACA

RORγT

Probe

/56-FAM/AGA AATGCC /ZEN/TTGCCG TAG GGATGT CT/31ABkFQ/

Forward

AACCGTTGCCAACACTGTCGATTG

Reverse

ACGCCATGCCACTCTACA ACT CAA

  1. FAM = 6-carboxyfluorescein; fluorophore; ZEN = 5′ quencher; ABkFQ = Iowa Black FQ; 3′ quencher.