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Figure 1 | Virology Journal

Figure 1

From: Virus replicon particle based Chikungunya virus neutralization assay using Gaussia luciferase as readout

Figure 1

CHIKV VRP system. (A) Schematic presentation of the CHIKV replicon expressing Gluc marker and the CHIKV helper-C and helper-E RNAs. Lines represent non-translated regions and boxes represent translated regions, whereas white boxes indicate nonstructural proteins and gray boxes indicate structural proteins. The Gluc reporter is represented as black box. In the helper constructs 5’ terminal nucleotides of the nsP1 gene important for RNA replication were retained. Arrows indicate the position of the subgenomic promoter. The solid black circle at the 5’ end of each RNA represents the CAP structure; (A)n indicates the poly(A) tail. (B) Kinetics of Gluc secretion after electroporation of CHIKV replicon expressing Gluc. In vitro-synthesized replicon RNA was electroporated into BHK cells and release of Gluc into the supernatant was measured at the indicated time points.

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