Figure 6
From: The N-end rule and retroviral infection: no effect on integrase
Intravirion integrase levels are decreased in HIV-1 integrase N-terminal mutants. HIV-1 or MLV virions were produced in 293T cells using helper plasmids with either WT or engineered mutations for the N-terminus of mature integrase. Virions particles were concentrated and capsid equivalent (p24 for HIV-1 and p30 for MLV) extracts were analyzed by immunoblot analysis. A) HIV-1 vector particle extracts probed with p24, integrase or RT antibodies. The MLV (ctrl) particle extract serves as a negative control. B) MLV vector particle extracts probed with p30 or MLV integrase antibodies. The HIV (ctrl) extract serves as a negative control.