Artificial microRNA-mediated resistance against the monopartite begomovirus Cotton leaf curl Burewala virus

Table 4 Infectivity of ToLCNDV in transgenic N. benthamiana plants harbouring amiRNA

Plant	Infectivity				Diagnostics
	(plants symptomatic/plants inoculated)				Diagnostics
	Exp. 1		Exp.2		PCR^£		Southern^$
	15dpi	28dpi	15dpi	28dpi	Exp. 1	Exp.2
N. b	10/10	10/10	9/10	10/10	10/10	10/10	++++
P1C	9/10	10/10	7/10	10/10	10/10	10/10	+++
P1D	10/10	10/10	10/10	10/10	10/10	10/10	++++
N. b*	0/10	0/10	0/10	0/10	0/10	0/10	−
N. b ^#	0/5	0/5	0/5	0/5	0/5	0/5	−

* Non-transgenic N. benthamiana plants inoculated with Agrobacterium cultures harbouring pGreen0029.
^# Non-inoculated plants.
^£ ToLCNDV was detected in nucleic acids extracted from plants by PCR using primers ToLCNDVV2F and ToLCNDVV2R (Table 5).
^$ Southern hybridization results are given as strong hybridization (++++), through weak (+) hybridization to no hybridization detected (−).

ISSN: 1743-422X