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Table 1 Infectivity of CLCuBuV in transgenic N. benthamiana plants harbouring amiRNA

From: Artificial microRNA-mediated resistance against the monopartite begomovirus Cotton leaf curl Burewala virus

Plant

Infectivity

Diagnostics

(plants symptomatic/plants inoculated)

Exp. 1

Exp.2

PCR£

Southern$

15dpi

28dpi

15dpi

28dpi

Exp. 1

Exp.2

 

N. b

8/10

10/10

10/10

10/10

9/10

10/10

++++

P1C

2/10

0/10

1/10

0/10

10/10

10/10

+

P1D

3/10

8/10

2/10

10/10

10/10

10/10

+++

N. b*

0/10

0/10

0/10

0/10

0/10

0/10

ND

N. b #

0/5

0/5

0/5

0/5

0/5

0/5

−

  1. * Non-transgenic N. benthamiana plants inoculated with Agrobacterium cultures harbouring pGreen0029.
  2. # Non-inoculated plants.
  3. £ CLCuBuV was detected in nucleic acids extracted from plants by PCR using primers IRVF and IRVR (Table 5).
  4. $ Southern hybridization results are given as strong hybridization (++++), through weak hybridization (+), to no hybridization detected (−). Some plants were not examined for the presence of virus by hybridization (ND).
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Virology Journal

ISSN: 1743-422X