Whole-genome analysis of piscine reovirus (PRV) shows PRV represents a new genus in family Reoviridae and its genome segment S1 sequences group it into two separate sub-genotypes

Table 3 Piscine reovirus (PRV) genome coding assignments and protein characteristics

Genome segment*	Molecular size (bp)**	5′UTR (bp)**	3′UTR (bp)**	Protein name***	Protein size (aa) & mass (kDa)¹	pI value	Predicted function
L1	3911	18	44	Core shell (T = 1) [λ1]	1282, 141.41 kDa	5.47	major inner capsid protein, Helicase, RNA triphosphatase
L2	3935	18	44	Core turret [λ2]	1290, 143.75 kDa	4.81	core spike, guanylyl transferase
L3	3916	7	48	Core RdRp [λ3]	1286, 144.24 kDa	8.68	minor inner capsid protein, RNA polymerase
M1	2383	21	79	Core NTPase [μ2]	760, 86.09 kDa	8.23	minor inner capsid protein, nucleoside triphosphate phosphohydrolase
M2	2179	26	89	Outer shell (T = 13) [μ1]	687, 74.26 kDa	6.27	outer capsid protein, membrane penetration, apoptosis
M3	2403	83	61	NS factory [μNS]	752, 83.53 kDa	5.00	NS, genome packaging?
S1	1081	28	60	Outer clamp [σ3]	330, 37.08 kDa	7.43	major outer capsid protein, dsRNA binding protein, translation control, modulation of cellular interferon, zinc-binding
S1	1081	28	60	NS, p13 [σ1s]	124, 12.99 kDa	4.88	NS, block cell-cycle progression, cytolytic in PRV
S2	1329	21	45	Core clamp [σ2]	420, 45.93 kDa	9.02	major inner capsid protein, morphogenesis?
S3	1143	28	50	NS RNA [σNS]	354, 39.07 kDa	7.76	NS, genome packaging?
S4	1040	38	54	Outer fiber [σ1]	315, 34.60 kDa	6.04	outer capsid protein (virus attachment), cell tropism, pathways of viral spread in-vivo, virulence

*Genome segment nomenclature used by Palacio et al.[2] for PRV.
**Values obtained from cDNA sequenced [2; this paper PRV strain 358]. The 3′UTR does not include the stop codon.
***Protein nomenclature used by Key et al.[10] for PRV. Homolog of Mammalian reovirus protein is given in square brackets.
¹PRV gene products are calculated from sequence data [2; this paper PRV strain 358].

ISSN: 1743-422X