RT-PCR amplification and sequencing strategy. (a) Schematic illustration of viral genome segments of piscine reovirus (PRV) RT-PCR targets for nucleotide sequencing. (b) Gel electrophoresis of amplified products obtained from the RT-PCR for Canadian PRV isolate 358. The PCR primers are listed in Additional file 5: Table S4. Lane M denotes Molecular weight marker.