Scatter plot of 45-Ct values of viral RNA from organs of individual birds at 2 days post inoculation (d.p.i.). Chickens were inoculated with different LPAIV strains and the presence of viral RNA in organs was examined at 2 d.p.i. by qPCR. The horizontal line represents the mean of 5 or 6 individual birds with SD. Triangles represent the 45-Ct value of individual birds. The data were expressed as Ct-45 values, which means that increased values in the figures indicate increased amount of viral RNA and when Ct-45 is equal to zero no viral RNA was detected. No viral RNA was detected in controls (Ct = 45). M-LPAIV H5N1, C-LPAIV H7N1 and H7N7: n=6. C-LPAIV H5N2 and H9N2: n=5.