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Table 1 Virus isolation and titrations in lungs on day 3 post challenge

From: An M2e-based synthetic peptide vaccine for influenza A virus confers heterosubtypic protection from lethal virus challenge

Immunogena

Virus isolation/total

Protection against challenge and virus titerb

(log10 EID50/ml±S.D.)

 

PR8

SwGD96

SwHLJ1

PR8

SwGD96

SwHLJ1

M2e-MAP+Freund

5/5

0/5

0/5

3.60±0.22

<1

<1

Freund

5/5

5/5

5/5

5.80±0.27

3.26±0.29

2.15±0.34

  1. a. 60 mice per group were injected twice subcutaneously with M2e-MAP plus Freund and Freund as control.
  2. b. Mice were challenged intranasally 2 weeks post 1st boost with 106EID50 of SwGD96, SwHLJ1 and 10LD50 of PR8 viruses. Virus titer of lungs was determined 3 days later in ECE. The values were calculated by the method of Reed-Muench and expressed as the mean log10 EID50/ml±S.D. The lower limit of detection of virus was 1.0 log10 EID50/ml.