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Table 2 Comparison of infection reporter assay and the traditional crystal violet antiviral assay

From: A quantitative infection assay for human type I, II, and III interferon antiviral activities

  Crystal violet assay VSV-DsRed2 assay
Antiviral incubation 24 hours, 67ul sample 24 hrs, 67ul sample
Infection wt virus, 24 hour incubation RFP reporter virus, 24 hour incubation
Fixation 4% PFA in 5% sucrose, 20 min  
Rinse 2x PBS  
Stain Addition of crystal violet, overnight incubation  
Scan Microplate reader at 570 nm Fluorescent microplate reader 485/620 or fluorescent scanner 555/580 nm.