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Table 1 Assay statistics and reproducibility

From: A quantitative infection assay for human type I, II, and III interferon antiviral activities

A. Fluorescent plate scanner and whole-well signal integration
   Sensitivity (LOD) units/ml Variability intra-assay COV (%) Variability inter-assay COV (%)
   Assay1 Assay 2 Assay 3 Assay 4
IFNα VSV-DsRed2 1.51 +/− 0.12 0.9 2.9 0.7 1.7 2.4
VSV-ZsGreen 2.07 +/− 0.94 1.8 8.6 5.5 3.3 16.0
IFNβ VSV-DsRed2 1.71 +/− 0.27 1.4 1.1 0.6 1.0 5.1
VSV-ZsGreen 2.57 +/− 0.52 1.9 4.1 2.2 3.2 7.9
B. Fluorescence/absorbance plate reader
   Sensitivity (LOD) units/ml Variability intra-assay COV (%) Variability inter-assay COV (%)
   Assay 1 Assay 2 Assay 3 Assay 4  
  VSV-DsRed2 1.29 +/− 0.34 4.0 1.7 1.9 5.7 5.7
IFNα VSV-ZsGreen 0.96 +/−0.18 1.2 1.1 1.7 2.5 3.6
  Crystal violet 4.09 +/− 0.43 17.5 14.0 7.3 8.6 21.9
  VSV-DsRed2 2.71 +/− 0.29 3.0 1.8 1.7 3.6 6.4
IFNβ VSV-ZsGreen 1.87 +/− 0.11 2.3 3.1 0.7 1.8 4.4
  Crystal violet 8.44 +/− 2.28 20.1 6.6 4.3 7.3 18.5
  1. Viral replication reporters and two methods of signal measurement were tested for the functional antiviral assays and calculated IC50 values statistically compared with the traditional crystal violet antiviral assay. Coefficients of variance were calculated with a minimum of four replicates. +/− values are 95% confidence intervals over all samples.