Table 1 Assay statistics and reproducibility
From: A quantitative infection assay for human type I, II, and III interferon antiviral activities
A. Fluorescent plate scanner and whole-well signal integration | |||||||
|---|---|---|---|---|---|---|---|
Sensitivity (LOD) units/ml | Variability intra-assay COV (%) | Variability inter-assay COV (%) | |||||
Assay1 | Assay 2 | Assay 3 | Assay 4 | ||||
IFNα | VSV-DsRed2 | 1.51 +/− 0.12 | 0.9 | 2.9 | 0.7 | 1.7 | 2.4 |
VSV-ZsGreen | 2.07 +/− 0.94 | 1.8 | 8.6 | 5.5 | 3.3 | 16.0 | |
IFNβ | VSV-DsRed2 | 1.71 +/− 0.27 | 1.4 | 1.1 | 0.6 | 1.0 | 5.1 |
VSV-ZsGreen | 2.57 +/− 0.52 | 1.9 | 4.1 | 2.2 | 3.2 | 7.9 | |
B. Fluorescence/absorbance plate reader | |||||||
Sensitivity (LOD) units/ml | Variability intra-assay COV (%) | Variability inter-assay COV (%) | |||||
Assay 1 | Assay 2 | Assay 3 | Assay 4 | ||||
VSV-DsRed2 | 1.29 +/− 0.34 | 4.0 | 1.7 | 1.9 | 5.7 | 5.7 | |
IFNα | VSV-ZsGreen | 0.96 +/−0.18 | 1.2 | 1.1 | 1.7 | 2.5 | 3.6 |
Crystal violet | 4.09 +/− 0.43 | 17.5 | 14.0 | 7.3 | 8.6 | 21.9 | |
VSV-DsRed2 | 2.71 +/− 0.29 | 3.0 | 1.8 | 1.7 | 3.6 | 6.4 | |
IFNβ | VSV-ZsGreen | 1.87 +/− 0.11 | 2.3 | 3.1 | 0.7 | 1.8 | 4.4 |
Crystal violet | 8.44 +/− 2.28 | 20.1 | 6.6 | 4.3 | 7.3 | 18.5 | |