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Table 3 Primers for amplification of 1.3, 1.2, and 1.1 fold over length HBV replication-competent clones

From: Inhibition of hepatitis B virus (HBV) gene expression and replication by HBx gene silencing in a hydrodynamic injection mouse model with a new clone of HBV genotype B

Primer

Position

Sequence

Referencea

hbv1.1LF

HBV 1658-1677

5′-aac tgc ag a aga gga ctc ttg gac ttt c-3′

AY220698.1

hbv1.2LF

HBV 1360-1379

5′-aac tgc ag a gta tac atc att tcc atg g-3′

AY220698.1

hbv1.3LF

HBV 1040-1058

5′-tgc aac tgc ag t gga tat cct gct tta atg-3′

AY220698.1

hbvLR

HBV 253-233

5′-gc t cta ga c tct gtg gta ttg tga-3′

AY220698.1

hbvSF

HBV 247-264

5′-ggc tag tct aga ctc gtg gtg g-3′

AY220698.1

hbvSR

HBV 1986-1969

5′-acc gag ctc tcg aat aga agg aaa gaa-3′

AY220698.1

hbv1.3 F

pGEM-T 4-39

5′-ata aga at g cgg ccg c cg aat tgg gcc cga cgt cg-3′

X65308.2

hbv1.3R

pGEM-T 117-81

5′-ata gtt ta g cgg ccg c gt tgg gag ctc tcc cat atg-3′

X65308.2

  1. Restriction sites are shown in italics. The gene sequences included in the primers are indicated by underling. a The Genbank accession numbers of the used reference sequences are given.