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Figure 5 | Virology Journal

Figure 5

From: Inhibition of hepatitis B virus (HBV) gene expression and replication by HBx gene silencing in a hydrodynamic injection mouse model with a new clone of HBV genotype B

Figure 5

The effect of HBx iRNA on HBV gene expression and replication in vitro. Different concentrations of HBx iRNA and pAAV-HBV1.3B were co-transfected into Huh-7 cells which were seeded in 24-well plates at approximately 60% confluence. The concentrations of the plasmids were indicated in the Tables. (A) HBsAg and HBeAg levels in the cell culture supernatants of cells co-transfected with 0.5 μg of pAAV-HBV1.3B- and 0.5 μg of pSR-HBxi314- or pSR-HBxi285. (B) HBsAg and HBeAg levels in the cell culture supernatants of cells co-transfected with pAAV-HBV1.3B and 0, 0.1, 0.3, or 0.5 μg of pSR-HBxi285. (C) HBV replicative intermediates in Huh-7 cells transfected with pAAV-HBV1.3B and 0, 0.1, 0.3, or 0.5 μg of pSR-HBxi285. (D) A comparison of the inhibition rate of HBsAg in the cell culture supernatants of cells co-transfected with pAAV-HBV1.3B- and pSB-HBxi285- or pSR-HBxi285.

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