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Figure 2 | Virology Journal

Figure 2

From: Inhibition of hepatitis B virus (HBV) gene expression and replication by HBx gene silencing in a hydrodynamic injection mouse model with a new clone of HBV genotype B

Figure 2

HBV replication and gene expression in vivo after HI with pAAV-HBV1.3 B or pAAV-HBV1.2. 10 μg of pAAV-HBV1.3B or pAAV-HBV1.2 were injected into the tail veins of C57BL/6 mice. pAAV-HBV1.3B group included 14 mice and pAAV-HBV1.2 group included 9 mice. (A) Real-time detection of HBV DNA in mouse sera at the indicated time points (the number of the mice ≥ 3). (B) Southern blot analysis with 20 mg of total DNA extracted from liver tissues of the mice HI with pAAV-HBV1.3B or pAAV-HBV1.2 at different time points after HI. All DNA samples were treated with RNase before subjected to agarose gel electrophoresis. Bands corresponding to the expected size of the input HBV plasmids, relaxed circular (RC) and single-stranded (SS) HBV DNAs are indicated. (C) Real-time PCR detection of HBV DNA levels in the liver of the mice after HI. (D) Immunohistochemical staining of the liver sections for HBcAg in hepatocytes of HBsAg-positive or HBsAg-negative mice at day 252 after HI with pAAV-HBV1.3B (1, HBsAg positive mice and 3, HBsAg negative mice) and at day 340 after HI with pAAV-HBV1.2 (2, HBsAg positive mice and 4, HBsAg negative mice) (Original magnification: 400X).

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Virology Journal

ISSN: 1743-422X