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Figure 9 | Virology Journal

Figure 9

From: Isolation and genetic characterization of human coronavirus NL63 in primary human renal proximal tubular epithelial cells obtained from a commercial supplier, and confirmation of its replication in two different types of human primary kidney cells

Figure 9

HCoV-NL63/RPTEC/2004 titers in cultured cells. [A]. Virus titers seven days post-infection of indicator cells infected with HCoV-NL63/RPTEC/2004 pp A. Titers were obtained from free virus in spent media; plaque assays were performed in CaCo-2 cells. Average virus titers (PFU/ml, mean of 3 measurements) were: LLC-MK2 cells, 3.2 × 105; Vero E6 cells, 2.3 × 104; HEK-293 cells, 5.9 × 104; CV-1 cells, 1.6 × 104; MDCK-NBL cells, 4.3 × 103; MDCK-London cells, 4.1 × 103; Mv1 Lu cells, 6.9 × 103; WI-38 cells, none detected. [B]. Virus production over nine days by NL63/RPTEC/2004 pp D in LLC-MK2 cells. Titers (PFU/ml) peaked on day 6, and remained in the low 105 range thereafter until day 9 p.i. (last day of measurement).

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Virology Journal

ISSN: 1743-422X