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Figure 5 | Virology Journal

Figure 5

From: Isolation and genetic characterization of human coronavirus NL63 in primary human renal proximal tubular epithelial cells obtained from a commercial supplier, and confirmation of its replication in two different types of human primary kidney cells

Figure 5

RT-PCR detection of HCoV-NL63 in LLC-MK2 cells. Lane M, 100 bp MW markers (New England Biolabs); Lane 1, HCoV-NL63-specific PCR product (314 bp) amplified by PCR primers N5-PCR1 and N3-PCR1 [33]; Lane 2, HCoV-NL63-specific PCR product (237 bp) amplified by PCR primers repSZ-1 and SZ-3[33]; Lane 3, Non-infected LLC-MK2 control tested using PCR primers N5-PCR1 and N3-PCR1; Lane 4, Non-infected LLC-MK2 control tested using PCR primers repSZ-1 and SZ-3.

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Virology Journal

ISSN: 1743-422X