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Figure 3 | Virology Journal

Figure 3

From: Maintaining the structural integrity of the bamboo mosaic virus3′ untranslated region is necessary for retaining the catalytic constant for minus-strand RNA synthesis

Figure 3

RNA stability and in vitro translation assays of BaMV-S and its derivatives in wheat germ extract. (A) RNA stability assay in wheat germ. RNAs were extracted from in vitro translation reaction at various incubation times as indicated and were subjected to a Northern blot analysis. (B) In vitro translation was performed in wheat germ extract with RNA of BaMV-S and its derivatives. The products were separated by 10% SDS-PAGE and analyzed by a phosphorimager (Fujifilm BAS 2500). The molecular masses of the markers are indicated on the left. The arrow indicates the position of the replicase.

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