Skip to main content
Figure 2 | Virology Journal

Figure 2

From: Maintaining the structural integrity of the bamboo mosaic virus3′ untranslated region is necessary for retaining the catalytic constant for minus-strand RNA synthesis

Figure 2

Analysis of BaMV protein and RNA accumulation of BaMV-S and its derivatives in N. benthamiana protoplasts. (A) Western blot analysis of BaMV coat protein (25 kDa) in protoplasts inoculated with the RNA transcripts (5 μg) indicated on the top of each lane. Total protein extracts were separated on a 12% SDS-polyacrylamide gel, blotted, and probed with a rabbit-anti BaMV coat protein antiserum, followed by a fluorescence-labeled anti-rabbit IgG antibody. The bolt was scanned and quantified afterwards. rbcL indicates the large subunit of RuBisCo stained with coomassie blue as a loading control. (B) Northern blot analysis of BaMV genomic (6.4 kb) and two subgenomic (2.0 and 1.0 kb) RNAs in protoplasts. The blot was probed with a 32P-labelled RNA complementary to a 600-bp area at the 3′-end of genomic RNA. The ribosomal RNA (rRNA) shown under the blot served as the sample loading control.

Back to article page

Virology Journal

ISSN: 1743-422X