Table 2 Oligonucleotide sequences of inverse PCR primers used to construct Myc PRMT6 and Tat-FLAG mutants
From: Overexpression of PRMT6 does not suppress HIV-1 Tat transactivation in cells naturally lacking PRMT6
Wild type | Mutant | Forward primera | Reverse primera |
|---|---|---|---|
Myc-PRMT6 | ∆N | acggtactggacgtggg | cgaattcccgttgttcag |
|  | ∆CD | gcctccgccgagctcttc | cttgcctcgcagtgctg |
|  | ∆C1 | cagcgctttgctcagcta | cggcaggagaagaccgc |
|  | ∆C2 | gcgctcctctacctgaac | tggacgagccagcacgt |
|  | ∆C3 | tgagaattcctgcagatatccag | ctgtttccagtgagtggcc |
Tat-FLAG | ∆16 | cagcctaaaactgcttgtacc | catggtggcaagcttaagt |
|  | ∆CC | aggaagaagcggagacag | agcagttttaggctgacttc |
|  | ∆B | cctcctcaaggcagtcagac | gccataggagatgcctaagg |
|  | ∆SE | ggaggcgattataaggacga | ttgctttgatagagaaactt |
| Â | EDE | tcctagactagcgccctg | gctactggcgccatgg |
| Â | CS | tcttcctttcattcccaagtttct | ctttttagaataggaattggtagaagca |