Figure 3From: Vaccination induced antibodies to recombinant avian influenza A virus M2 protein or synthetic M2e peptide do not bind to the M2 protein on the virus or virus infected cellsMeasurement of antibodies to avian influenza in serum with the IDEXX AI Flockcheck Test (IDEXX). Vaccination groups are indicated on the X-axis by immunogen present in the vaccine (M2 Protein = E. coli recombinant his-tagged M2 protein; M2e Peptide = synthetic M2e peptide, M2e Tetramer = synthetic biotinylated M2e peptide conjugated with Streptavidin, HSP70 Tetramer = synthetic biotinylated HSP70 control peptide conjugated with Streptavidin). The average value of the sample-negative/positive–negative (S/P) ratio +/− SEM from five animals in each group is shown. In addition the average value of the sample-negative/positive–negative (S/P) ratio +/− SEM from triplicates of a negative and positive reference sample from naive and H9N2 vaccinated chickens is shown (GD Deventer, The Netherlands). The threshold value of 0.5 for a positive S/P ratio as per manufacturer instruction is depicted as a dotted line.Back to article page