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Figure 3 | Virology Journal

Figure 3

From: Vaccination induced antibodies to recombinant avian influenza A virus M2 protein or synthetic M2e peptide do not bind to the M2 protein on the virus or virus infected cells

Figure 3

Measurement of antibodies to avian influenza in serum with the IDEXX AI Flockcheck Test (IDEXX). Vaccination groups are indicated on the X-axis by immunogen present in the vaccine (M2 Protein = E. coli recombinant his-tagged M2 protein; M2e Peptide = synthetic M2e peptide, M2e Tetramer = synthetic biotinylated M2e peptide conjugated with Streptavidin, HSP70 Tetramer = synthetic biotinylated HSP70 control peptide conjugated with Streptavidin). The average value of the sample-negative/positive–negative (S/P) ratio +/− SEM from five animals in each group is shown. In addition the average value of the sample-negative/positive–negative (S/P) ratio +/− SEM from triplicates of a negative and positive reference sample from naive and H9N2 vaccinated chickens is shown (GD Deventer, The Netherlands). The threshold value of 0.5 for a positive S/P ratio as per manufacturer instruction is depicted as a dotted line.

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