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Figure 1 | Virology Journal

Figure 1

From: Vaccination induced antibodies to recombinant avian influenza A virus M2 protein or synthetic M2e peptide do not bind to the M2 protein on the virus or virus infected cells

Figure 1

MBP-M2 protein specific antibodies in chicken sera obtained at the time of vaccination, three weeks after vaccination and two weeks after booster vaccination were determined by ELISA. The ELISA plate was coated with 0.5 μg/well affinity purified E. coli recombinant MBP-M2 protein. Vaccination groups are indicated on the X-axis by antigen present in the vaccine (M2 Protein = E.coli recombinant his-tagged M2 protein; M2e Peptide = synthetic M2e peptide, M2e Tetramer = synthetic biotinylated M2e peptide conjugated with Streptavidin, HSP70 Tetramer = synthetic biotinylated HSP70 control peptide conjugated with Streptavidin). Average OD 405 nm value +/− SEM from five animals in each group is shown. * = significant difference between indicated group and the same group before vaccination.

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Virology Journal

ISSN: 1743-422X