Figure 6
From: HeLa cell response proteome alterations induced by mammalian reovirus T3D infection
Western blot validation of host protein regulation. A, HeLa cells were mock-infected or infected for 24 h, or B, for indicated periods of time, harvested and lysed with 0.5% NP-40 detergent. The cytosolic and nuclear fractions were separately purified, dissolved in SDS electrophoresis sample buffer, and proteins resolved in 10% (A), or in 4-16% gradient (B) SDS-PAGE, transferred to PVDF, and probed with indicated antibodies. Antibody binding was detected with HRP-conjugated secondary antibodies and ECL, and visualized with an Alpha Innotech FluorChemQ MultiImage III instrument. Molecular weight standards are indicated at left and SILAC-measured ratios are indicated on right in A. *: not detected in indicated fraction; †: based on single peptide only.