Figure 8
From: A novel dengue virus detection method that couples DNAzyme and gold nanoparticle approaches
Analysis of DDZ-AuNP sensitivity. A) DDZ-M-AuNP colorimetric assays were performed on DENV-2 NGC titers of 1 × 101, 1 × 102, 1 × 104, and 1 × 106 to determine the limits of detection. Samples were assembled as described in Methods, incubated at 37°C for 30 minutes and photographed. Results show that the DDZ-M-AuNP colorimetric assay is capable of detecting DENV-2 at a titer as low as 1 × 101. DDZ-M = anti-dengue virus DNAzyme, DDZin-M = inactive anti-dengue virus DNAzyme. B) Analysis of DDZ-AuNP sensitivity by UV/Vis spectrophotometry. Ten microliters (10 uL) of cell culture fluid from Mock, DENV-2 NGC (titers of 1 × 101, 1 × 102 1 × 106/mL), or CHIKV vaccine strain 181/25 (1 × 106/mL) infected C6/36 cells, or DENV serially diluted from 1 × 101 (Dil1 through Dil5) or were added to separate mixtures as described in A). UV/Vis spectrophotometric analysis was performed using the ND-1000 spectrophotometer at an absorbance of 520 nm. Absorbance measurements were graphed in log scale to illustrate sensitivity and accuracy of the colorimetric DENV detection method.