Figure 4
From: A novel dengue virus detection method that couples DNAzyme and gold nanoparticle approaches
Colorimetric DDZ-AuNP detection of a synthetic DENV-2 NGC RNA target. A synthetic stretch of ribonucleotides corresponding to the 5’ 170 bases of the DENV-2 RNA genome was added to a buffered mixture containing 10 mM MgCl2, 1 × 105 DDZ-M-AuNP particles/mL, and 1.0 M NaCl. Samples were incubated at 37°C for 5 minutes and photographs were taken. Control samples were treated the same as experimental except 50 mM Tris–HCl was added in lieu of the synthetic DENV-2 RNA. Aggregation of DDZ-M tethered AuNPs only occurred in the presence of synthetic DENV RNA. Results indicate that DDZ-AuNPs have the ability to detect DENV. DDZ = anti-DENV DNAzyme.