Figure 3
From: A novel dengue virus detection method that couples DNAzyme and gold nanoparticle approaches
In vitro DDZ activity assay. DDZ-M-AuNPs (1 × 105 particles/mL) were placed in a buffered solution containing 10 mM MgCl2, DENV-2 NGC RNAs (0.6 μM) isolated from Ae. albopictus C6/36. Following incubation at 37°C for 30 minutes, RT-PCR was performed as described in Materials and Methods to amplify digestion products. Fragments were then separated on a 1.75% TAE agarose gel in the presence of ethidium bromide and photographed under UV light. Arrows indicate digestion products of approximately 150 and 350 bases and the primer dimer, respectively. Results demonstrate DDZ-M digestion of full length DENV-2 genome in spite of AuNP conjugation. DDZ = anti-dengue virus DNAzyme, DDZin = inactive anti-dengue virus DNAzyme.