Figure 2

Delineation of the amino acid residues within the N-terminus of BBSV CP involved in RNA binding with North-western blot analysis. (A) Confirmation of the quantity and purity of wild type (WT) and mutated BBSV CPs prepared with an E. coli expression system using SDS–PAGE (top) and Western blot (bottom). GFP was used as a control. (B) RNA binding capability of WT and mutant CPs were assayed using non-radioactively labeled BBSV RNA (see Materials and Methods for details). The experiment was repeated three times with similar results.