Table 1 PCR primers and probes
From: No association between XMRV or related gammaretroviruses in Australian prostate cancer patients
Target | Amplicon Size | Method | Primers and TaqMan Probe (5’-3’) | |
|---|---|---|---|---|
HuVAMP21 | 78 bp | qPCR | HuVAMP2-F | CAGCATCTCTCCTACCCTTTCAC |
HuVAMP2-R | CCCCACACTTCTGGTTTTCTG | |||
Huvamp2-Probe | 6FAM-AGCAGGGATATCTAAGC-MGBNFQ2 | |||
BMLV3 | 76 bp | qPCR | BMLV-F | GCCTGTCCAGGATCTGAGAG |
BMLV-R | GAGGTTGTAAGGGTTGGGCA | |||
BMLV- Probe | 5FAM-AAGTCAACAAGCGGGTGGAAGABHQ4 | |||
XMRVIN5 | 70 bp | qPCR | XMRVIN-F | CGAGAGGCAGCCATGAAGG |
XMRVIN-R | GCGTATACGGGGTTGAGTCC | |||
XMRVIN-Probe | 6FAM-AGTTCTAGAAACCTCTACACTC-MGB | |||
IAP6 | 71 bp | qPCR | MIAP-F | GCCGCGCCCACATT |
MIAP-R | CGCAGATTATTTGTTTACCACTTAGAA | |||
MIAP-Probe | 6FAM-CCGTTACAAGATGGTGCTGA-MGBNFQ | |||
RNASEL | 137 bp | PCR | 462R-F | GTGGAAAATGAGGAAGATGAATTTGCCAG |
462Q-F | GTGGAAAATGAGGAAGATGAATTTGCCAA | |||
462-R | ATTGGGGACTCACCTATTAAGATGTTTTG | |||