Figure 6
From: Examination of soluble integrin resistant mutants of foot-and-mouth disease virus
O1 Campos derived SIR mutants exhibit distinctive alterations. A. Schematic of O1 Campos derived O-SIR mutant production. B. Comparison of the growth characteristics of O-SIRs relative to O1 Campos after ssαvβ6 co-incubation on LFBK and CHO K1 cells. O-SIR #9 was the only virus that amplified on CHO 677 cells. Titers represent PFU/mL. C. WT and O-SIR viruses were co-incubated with ssαvβ6 on LFBK monolayers for 24 h, and the calculated titers were subsequently plotted. D. RIP analysis of untransfected LFBK and COS-1 cells infected with: O1 Campos and O-SIRs in the presence of 35S-methionine. NT, not transfected (COS-1). PC, positive control (LFBK). E. FMDV genome depicting amino acid substitutions identified in the P1 region of O-SIR mutants.