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Figure 6 | Virology Journal

Figure 6

From: A new reporter mouse cytomegalovirus reveals maintained immediate-early gene expression but poor virus replication in cycling liver sinusoidal endothelial cells

Figure 6

Live cell imaging and single cell analysis of LSEC-uniLT infected with the MIEPr. Cells were infected at an MOI of 10 and monitored by fluorescence microscopy in a CO2 regulated chamber for 5 days. (A) Representative time courses of fluorescence intensities for YFP and tdTomato (Tom) in individual LSEC-uniLT. The signal intensity is represented on the ordinate, whereas the time post infection is shown on the abscissa. (B) The median fluorescence intensity ratio of tdTomato/YFP was established for individual infected cells and plotted against the survival time of the infected cell. (C) LSEC-uniLT shown in panel B were subdivided into terciles according to their median tdTomato/YFP-ratio. Symbols show survival times of individual cells in the lower (1), centre (2) and upper tercile (3), while horizontal lines show group average (+/- SEM) values. * and ** denote p values below 0.05 and 0.0035, respectively, according to Kruskal-Wallis followed by Dunns post-analysis test.

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Virology Journal

ISSN: 1743-422X