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Figure 4 | Virology Journal

Figure 4

From: A new reporter mouse cytomegalovirus reveals maintained immediate-early gene expression but poor virus replication in cycling liver sinusoidal endothelial cells

Figure 4

Cell proliferation has no effect on the early reporter gene expression of the MIEPr. (A) LSEC-uniLT (LSEC) were cultured in the presence (+ DOX) or absence (- DOX) of doxycycline. Cells were infected with an MOI of 10 and analyzed at 0, 4, 6, 12 and 24 hpi by flow cytometry. Representative dot blots of tdTomato and YFP expression are shown. (B) LSEC-uniLT were cultured in the presence (+DOX) or absence (-DOX) of doxycycline and infected with an MOI of 1 or left uninfected (control). At day 5 post infection confocal images were acquired to visualize cells by differential interference contrast (DIC) and the fluorescent reporters (YFP, tdTomato) by fluorescence imaging.

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