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Figure 1 | Virology Journal

Figure 1

From: A new reporter mouse cytomegalovirus reveals maintained immediate-early gene expression but poor virus replication in cycling liver sinusoidal endothelial cells

Figure 1

Growth and susceptibility to MCMV infection of the conditionally immortalized liver sinusoidal endothelial cell line LSEC-uniLT (LSEC). (A) 103 LSEC-uniLT were cultured for 10 days in 10 cm Petri dishes in the presence of 2 μg/ml doxycycline (+DOX) or in its absence (-DOX). Representative crystal violet stains visualizing cell proliferation are shown. (B) Representative brightfield microscopy picture of confluent LSEC-uniLT is shown. (C) LSEC-uniLT were infected at 0.1 MOI of MCMV in presence or absence of doxycycline and virus growth was monitored by plaque assay of supernatants at indicated time-points post infection. The average PFU/ml +/- SD of three experiments is shown. The limit of detection (DL) is marked by the dashed line.

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Virology Journal

ISSN: 1743-422X